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KMID : 0900220160230010017
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대한비과학회지
2016 Volume.23 No. 1 p.17 ~ p.23
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Effect of High-Insulin on MUC4, MUC5AC, and MUC5B Expression in Airway Epithelial Cells
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Na Hyung-Gyun
Bae Chang-Hoon
Choi Yoon-Seok
Song Si-Youn
Chin Hyun-Jung
Kim Yong-Dae
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Abstract
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Background and Objectives: Insulin is a peptide hormone that regulates the metabolism of carbohydrates and fats by
promoting the absorption of glucose from the blood to skeletal muscles. Insulin has been reported to be closely related to cardiovascular, respiratory, and endocrine disease. However, the effect of insulin on production of major mucins in human airway epithelial cells has not been reported. Therefore, this study investigated the relationship between high levels of insulin and mucin in human airway epithelial cells.
Materials and Methods: This study analyzed the effect of high level of insulin on MUC4, MUC5AC, and MUC5B expression
using reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay in human airway epithelial cells.
Results: In human NCI-H292 airway epithelial cells, high level of insulin significant increased MUC4, MUC5AC, and
MUC5B mRNA expression and glycoprotein production. In the primary cultures of normal nasal epithelial cells, high level of insulin also increased MUC4, MUC5AC, and MUC5B expression.
Conclusion: These results suggest that insulin plays a role in control of mucus hypersecretion in human airway epithelial
cells.
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KEYWORD
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Insulin, MUC4, MUC5AC, MUC5B, Human airway epithelial cell
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